reverse transcription PCR
- 网络反应;反转录聚合酶链反应;反转录聚合酶链式反应
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Detection of Hepatitis A Virus by Two Reverse Transcription PCR Approaches
两种反转录聚合酶链反应检测甲型肝炎病毒
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Application of in situ reverse transcription PCR techniques
原位反转录PCR技术的应用
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Application of reverse transcription PCR in the detection of respiratory syncytial virus infection
反转录-聚合酶链反应在呼吸道合胞病毒感染检测的应用
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Rapid identification of subtypes of respiratory syncytial virus by real-time reverse transcription PCR
实时逆转录聚合酶链反应快速鉴别呼吸道合胞病毒亚型
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CDNA-AFLP differential patterns for 5 selected genes were confirmed via real-time reverse transcription PCR analysis .
用荧光实时定量PCR分析法证实了cDNA-AFLP基因表达结果。
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The gene expression of a-actin mRNA were determined by using the quantitative reverse transcription PCR technology .
利用半定量反转录聚合酶链式反应(QRT-PCR)法测定骨骼肌α-actin基因表达。
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Identification of Viruses Causing Respiratory Tract Infection by Single-tube Multiplex Reverse Transcription PCR
应用多重反转录PCR技术检测病毒性呼吸道感染
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A reverse transcription PCR system was established for the detection of Noroviruses in shellfish .
本文建立了贝类产品中诺沃克病毒检测的普通RT-PCR方法。
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A coupled one-step reverse transcription PCR procedure for cloning the human augmenter of liver regeneration gene
RT-PCR一步法克隆人肝再生增强因子基因
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A Reverse Transcription PCR that Distinguishes the Isolates of Low Virulent Strains From Virulent Strains of Newcastle Disease Virus
鸡新城疫病毒强弱毒株RT-PCR鉴别诊断方法
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Real-time fluorescence quantitative reverse transcription PCR in determination of guanylyl cyclase-C gene expression in peripheral blood of patients with colorectal cancer and its clinical significance
实时荧光定量PCR检测大肠癌患者外周血鸟苷酰环化酶C基因及其临床意义
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Reverse transcription PCR and in situ hybridization were used to detect the expression changes of CaBP mRNA in the brain of PD and control mice .
用反转录PCR及原位分子杂交方法检测PD鼠和正常鼠脑内CaBPmRNA的表达变化。
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The silencing effects of TIMP-1 on the mRNA and protein levels were examined by reverse transcription PCR ( RTPCR ) and Western blot analysis respectively .
采用RT-PCR和Western印迹测定宿主细胞TIMP-1在基因和蛋白水平的沉默效果。
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Recombinant human EGF was used to stimulate HepG_2 cells and semi-quantitative reverse transcription PCR was adopted to detect the expression of VEGF in HepG_2 cells .
离体实验中,用重组人EGF刺激人肝癌细胞系HepG2,采用半定量逆转录PCR检测VEGF的表达情况。
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Methods The interested segment was obtained by reverse transcription PCR with the designed specific primers , and inserted into pMD-18T vector by T / A match .
方法设计特异引物,用RT-PCR方法从乳腺癌组织扩增获得目的片段,利用T/A克隆将PCR产物插入pMD-18T载体;
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The method of reverse transcription PCR ( RT-PCR ) is more sensitive and reliable than that of enzyme-linked immunosorbent assay on nitrocellulose membranes ( NCM-ELISA ) .
对于甘薯病毒病的检测,反转录PCR法(RT-PCR)比硝化纤维素膜酶联免疫吸附检测法(NCM-ELISA)更灵敏和可靠。
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Methods Techniques of cell culture in vitro and RT PCR ( reverse transcription PCR ) were used , gene expressions of p53 and fas in 4 tumor cell lines were measured after treated with ethanol extractive and water extractive .
方法采用肿瘤细胞体外培养技术及RT-PCR技术,观察阿魏菇不同剂量的水提物及醇提物对体外培养的四种肿瘤细胞p53及fas基因表达的影响。
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The rats of group B were treated with EPO after globe ischemia-reperfusion . Brain tissues were taken out after execution at different time point . The expression of caspase-9 mRNA was detected with reverse transcription PCR ( RT-PCR ) technique .
在相应时间点断头取脑,逆转录聚合酶链反应技术检测大鼠皮质caspase9mRNA的表达,并与对照组(C组)比较。
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Methods : Total RNA were extracted from human peripheral blood , and reverse transcription PCR ( RT PCR ) were used to amplify the whole gene sequences of HLA DR loci , and the PCR products were linked to vector and transformed to E.
方法:提取人外周血总RNA,RTPCR扩增HLADR等位基因全序,连接、转化大肠杆菌后并测序。
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Consequently , the positive ESTs which significantly correlate with lipidosis trait of goose were screened by Delta Differential Display Reverse Transcription PCR ( DDRT-PCR ) method and the high homologue relative genes were found by cloning , sequencing and homology analysis .
采用δ差异显示法筛选出与鹅脂肪沉积性状显著相关的阳性EST,进行了克隆测序和同源性比对分析,找出了高度同源性的相关基因。
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Methods Total RNA was extracted from peripheral blood lymphocytes ( PBL ) of patients with glioma and subjected to reverse transcription PCR . Variable region genes ( Vk and VH gene ) were amplified from the cDNA by using nested polymerase chain reaction .
方法从脑胶质瘤患者外周血淋巴细胞(PBL)中提取细胞总RNA,经逆转录后用套式多聚酶链反应(PCR)分别扩增抗体轻链VK和重链VH基因。
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Reverse transcription PCR analyses show that the goose PRLR mRNA is widely expressed in testis , seminal duct , ovary , oviduct , kidney , large intestine , and small intestine and the highest abundance are presented in kidney , oviduct , large intestine and small intestine .
RT-PCR结果表明,鹅PrlRmRNA在成年鹅睾丸、输精管、卵巢、输卵管、肾、大肠、小肠、脾组织中均有表达,其中以肾、睾丸、大肠及小肠中表达最为丰富。
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Method : The cardiac tissue was taken from 30 cadavers of CHD and 20 cadavers of non CHD . Primers were derived from RV gene encoding sequence of the enveloped protein E 1 which was highly conserved in rubella virus by reverse transcription PCR in cardiac tissue .
方法:以RV特异的糖蛋白E1编码的基因保守序列设计引物,采用RT-PCR技术,对30例CHD及20例非先天畸形(对照组)尸体的心肌组织标本进行RV基因的检测。
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Diagnosis of Infectious Bursal Disease Virus by Reverse Transcription Nested PCR
反转录&套式PCR方法检测鸡传染性法氏囊病病毒
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Clone of EPO Gene from Human Fetal Liver cDNA by Reverse Transcription and PCR
人胎肝cDNA中EPO基因的克隆
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An one step procedure was developed using the PCR ( polymerase chain reaction ) for the detection of both hepatitis A virus and poliovirus RNA , in which reverse transcription and PCR were processed in one tube .
建立的一步PCR方法即反转录和PCR在同一管中进行,同时检测甲型肝炎和脊髓灰质炎病毒病毒RNA。
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Methods : The putative envelope protein 2 gene fragments of HCV in sera from Shandong ( SD ) , Shanghai ( SH ) patients were amplified by reverse transcription nested PCR and sequenced .
方法:对来自山东(SD)、上海(SH)两地患者血清RNA进行逆转录-巢式PCR,扩增HCV囊膜蛋白2基因片段,用PCR直接测序法对该片段进行序列测定。
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Then , RT-PCR was carried out for IL-10 gene screening using total RNA as a template , specific primers which derived from GenBank data of hIL-10 as a primer for reverse transcription and PCR primers .
以总RNA为模板,根据人IL-10在GenBank中的全长开放读框设计的特异性引物进行反转录,RT-PCR进行IL-10基因筛选。PCR产物经酶切分析和DNA序列测定后;
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Methods Both real-time fluorescence quantitative RT-PCR and reverse transcription specific primers PCR was applied to analyze serum HCV RNA copy numbers and genotypes .
方法用实时荧光定量RTPCR和逆转录型特异性引物PCR同时检测21例费城酒精依赖丙肝患者的血清HCVRNA拷贝数并分析HCV基因型。
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We primarily studied the differential expressions of arabidopsis thaliana seedlings under sound stimulation by mRNA reverse transcription differential display PCR ( DDRT-PCR ) with silver staining in this thesis .
本文运用银染mRNA逆转录差异显示(DDRT-PCR)技术,初步研究了声波刺激对拟南芥基因表达差异的影响。